Elucidation of total phenolic content and antioxidant activity in medicinal Aroid, Alocasia longiloba Miq.

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Abstract

Alocasia longiloba Miq belongs to Araceae family, believed to have the medicinal potential. However, the scientific evidences on this plant were limited. Therefore, this study aimed to evaluate the total phenolic content (TPC) and antioxidant activity of the different extracts (methanol, ethyl acetate and hexane) of A. longiloba leaf blades. The TPC was investigated by Folin-Ciocalteu method and antioxidant activity was determined by DPPH scavenging assay. The results showed ethyl acetate extract had the highest content of phenolic with 46.013 mg GAE/g followed by methanol extract (32.936 mg GAE/g) and hexane extract (31.782 mg GAE/g). The hexane extract exhibited the highest DPPH antioxidant acitivity, followed by ethyl acetate and methanol with IC ₅₀ values 2.519 µg/mL, 2.758 µg/mL and 9.542 µg/mL respectively. The results indicate A. longiloba has natural sources of antioxidant that can be used for the medicinal purposes.

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Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria

Gislene Nascimento, Juliana Locatelli, Paulo Freitas … (2000)

The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm), Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus and Syzygyum joabolanum, which inhibited 64.2 and 57.1% of the tested microorganisms, respectively, with higher activity against antibiotic-resistant bacteria (83.3%). Sage and yarrow extracts did not present any antimicrobial activity. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibiotic-resistant bacteria. The results obtained with Pseudomonas aeruginosa was particularly interesting, since it was inhibited by clove, jambolan, pomegranate and thyme extracts. This inhibition was observed with the individual extracts and when they were used in lower concentrations with ineffective antibiotics.